Objective: The purpose of this study were to produce and characterize crude fucoidan from brown sewed (Sargassum aquifolium), formulate and characterize the crude fucoidan-chithosan nanoparticle with ionic gelation method.
Methods: The crude fucoidan was produced by extraction using etanol 80% (v/v), digestion using calcium chloride solution 2% (w/v) and freeze drying to obtain brown seaweed’s dry extract. Nanoparticles were formed by ionic gelation method by mixing the brown seaweed extract and chitosan at the same concentration of 0.2% in ratio of (3:1). The characterization of crude fucoidan were done using FTIR and LC-MS, while the nanoparticle characterization include the determination of its particle size, polydispersity index, zeta potential and morphology.
Results: The characterization of crude fucoidan from brown seewed showed the molecular weight of 7665.57 Da and spectrum that is similar to the spectrum of the reference fucoidan. The nanoparticle of crude fucoidan and chitosan was showed the average of particles diameter of 326.6 nm, polydispersity index of 0.287, average of zeta potential of 54.23 mV, and the particles morphology were spheris.
Conclusion: The crude fucoidan from brown seewed (Sargassum aquifolium) showed similar characteristics with reference fucoidan, and the characteristics of crude fucoidan chitosan nanoparticles had fulfill the requirements of good nanoparticles.