Cyclea barbata L. Miers is a plant that has been traditionally utilized by Indonesian community as a refreshing drink, colds, fever and anti inflammation. The pirposed of the study was standardized of 70% ethanol extract and the antioxidant activity using DPPH method from Cyclea barbata L.Miers leaves. The characteristic of 70% ethanol extract showed the non specific parameters has brown viscous extract, bitter taste and aromatic odor, water content of 6.07± 0.07%, loss on drying of 7.47±0.28%, total ash content of 8,30±0.1%, total acid insoluble ash content of 0.12±0.0%, ethanol residual content of 0.70±0.16%, heavy metal contaminant (Cd: 0.002 mg/kg, Pb 0.03 mg/kg), total number of bacteria of 2,94 x 103 colony/g, and fungi (molds/yeasts) of 8.63 x 102 colony/g, ethanol residual content of 0.70±0.16%, whereas characteristic of specific parameter showed dilute ethanol content of 71.34±0.72%, dilute water content of 67.14±0.17% and assay of total flavonoid of 9.93±0.2%, the phytochemical screening was estimated that revealed the presence alkaloids, flavonoids, steroids/triterpenoid, tannin, saponins, and coumarins. The ethanol extract of Cyclea barbata leaves contain flavonoid as antioxidant activity with IC50 value of 49.45±0.64 pg/mL. The ethanol extract of Cyclea barbata leaves meets requirements standards that include specific and non specific parameter as a raw material for medicine.