Kelor ( Moringa oleifera Lamk) has been used empirically as medicinal plant which is herbaceous that can be grown easily on tropical land. According to literature this plant containing highly nutrients such as mineral, amino acid, protein, fat, vitamin A , B1, B2, B3 and C. This research aimed to obtain secondary metabolite data, biological activity with BSLT method and antioxidant activity with DPPH free radical scavenging from kelor leaves and their extract. The extraction was carried out by maceration with ethanol 70 % (v/v) and partitioned gradient started with n - hexan and being followed with ethyl acetate and n- buthanol. Results from the phytochemical screening of powder of kelor leaves and the extract showed that the powder contained alkaloid, flavonoid, saponine, tannin, steroid, triterpenoide, and coumarine, whereas n-hexane extract contained steroide, triterpenoide and ethyl acetate extract contained alcaloide, flavonoide, tannin, steroide, triterpenoide. and coumarine Furthermore, n- buthanol extract contained flavonoide and tannin Based on test using BSLT method, the highest activity was shown by n- buthanol extract with LC 50 of 32 ppm. In addition, ethyl acetate extract showed the highest antioxidant activity with IC 50 of 107,73 ppm. As conclusion, kelor leaves have a cytotoxic activity and are potential as antioxidant agent.