Objective: The purpose of this research was to optimize and validate HPLC method for determining the curcumin content of tablet preparations in less than ten minutes.
Methods: Curcumin can be analyzed qualitatively or quantitatively using reverse phase HPLC equipped with an isocratic elution system. Furthermore, this research used a visible detector, which contained octadecyl (C18) in its stationary phase at a wavelength of 428 nm and a mobile phase of acetonitrile-acetic acid-aquabides (60:1:39) mixture with a flow rate of 1.0 ml/minute. The chromatographic setup can separate curcuminoids, such as bisdemethoxycurcumin, demethoxycurcumin, and curcumin in less than 10 min. The method is valid on the basis of its selectivity, linearity, accuracy, and precision.
Results: The results showed that there was a high degree of selectivity with a resolution value (Rs) of 1.670 and a high degree of linearity with a correlation coefficient (r) of 0.9915 over a range of 1.38-2.04 μg/ml. Curcumin had a recovery test value of 100.73±1.04%. The curcumin content of the tablet preparations by producing A and B were 3.99±0.02% and 3.97±0.01%, respectively.
Conclusion: According to the results, HPLC is a valid method for determining curcumin levels in tablet preparations in accordance with ICH specifications (International Conference on Harmonization).